Rox Prothrombin

REF 200040

For quantitative determination of Prothrombin (FII) functional activity in plasma and FII containing concentrates. The method is suitable for plasma collected in citrate or EDTA.

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- A chromogenic Prothrombin method based upon the prothrombinase complex.

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- non-γ-carboxylated-FII is not activated in the method in contrast to snake venom based prothrombin methods.

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- 1 Kit = 4 x 25 test

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- Detection Limit = about 0.05 U/mL (5%) when using a plasma dilution of 1:200 as   prescribed in the package insert. The detection limit for undiluted samples is about 0.25 mU/mL.

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Measurement Principle

FII functional activity is determined in a chromogenic prothrombinase method, in which human FII is activated to thrombin (FIIa) by human FXa in the presence of bovine FV, calcium ions and phospholipid.

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The amount of FIIa formed is determined from the hydrolysis of a chromogenic FIIa substrate. The FII activity of the sample is assigned vs. plasma or a FII concentrate standard with FII potency expressed in International Units (IU).

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The prothrombinase complex is sensitive to g-carboxylation and therefore non-γ-carboxylated-FII is not activated in this method in contrast to snake venom based prothrombin methods.

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